Efek Polimorfisme Gena Nitrit Oksida Sintase3(nos3) Terhadap Kadar Nitrit Oksida Dan Tekanan Darah Pada Individu Terpapar Plumbum (the Effect of Polymorphisms Gene Nitric Oxide Synthase3 (Nos3) to Nitric Oxide Level and Blood Pressure on Lead-exposed Men)

摘要

The aim of these research were to identify the effect of polymorphisms gene NOS3 to nitric oxide level and blood pressure on lead-exposed men. The research used survey method and case control design. These cases of subject were 30 autorepair workers and 30 subject control from village as non polluted area. The genotype of individu investigated by Polimerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphisms (RFLP). The main parameters were nitric oxide (NO), blood pressure systole, dyastole and blood lead level. The data were analyzed by t test independent. These result showed that 40 % of cases subject were detected as individual polymorphism of gene NOS3 with GA alel. In contrast 60 % of cases subject and control subject were detected as non individual polymorphism with GG alel. Result t test for three parameters i.e. NO, systole, diastole blood pressure and lead showed highly significant difference (p=0) between individual polymorphism and non polymorphism. These research can be concluded that polymorphism of gene NOS3 on lead-exposed men influence bioability of NO, systole and diastole blood pressure. Blood lead level, systole and diastole of individual polymorphism are higher than individual polymorphism. The risk of hypertension are more seriously on polymorphisms lead-exposure worker than non polymorphisms.